Comparative table of HE conventional staining and Fast Staining | ||||||||||
HE Conventional staining program | HE fast staining program | |||||||||
Step | Specification | Time (S) | Mark | Step | Specification | Time (S) | Mark | |||
1 | Heating | 75℃-85℃ heating | 300 | Normal | 1 | Heating | 85℃ heating | 60 | Normal | |
2 | dewaxing | dewaxing solution | 300 | 2 | dewaxing and Hydration | Compound dewaxing solution | 120 | |||
3 | dewaxing solution | 300 | ||||||||
4 | Hydration | Ethanol | 60 | |||||||
5 | Ethanol | 60 | ||||||||
6 | 95% ethyl alcohol | 60 | ||||||||
7 | 85% ethyl alcohol | 60 | ||||||||
8 | Washing | water rinsing | 60 | 3 | Washing | water rinsing | 30 | |||
9 | Staining | Hematoxylin Stain | 360 | Priority | 4 | Staining | Hematoxylin Stain | 180 | Priority | |
10 | water rinsing | 120 | Normal | 5 | water rinsing | 240 | Normal | |||
11 | differentiation | 2 | Precise | |||||||
12 | water rinsing | 120 | Normal | |||||||
13 | Blue Promoting Solution | 10 | Precise | |||||||
14 | water rinsing | 120 | Normal | |||||||
15 | 95% ethyl alcohol | 60 | Normal | |||||||
16 | Eosin stain | 30 | Priority | 6 | Eosin stain | 30 | Priority | |||
17 | Dehydration | 85% ethyl alcohol | 30 | Normal | 7 | Dehydration and Transparent | 85% ethyl alcohol | 10 | Normal | |
18 | 95% ethyl alcohol | 60 | 8 | Compound transparent solution | 180 | Normal | ||||
19 | Ethanol | 120 | ||||||||
20 | Ethanol | 120 | ||||||||
21 | Transparent | transparent solution | 120 | |||||||
22 | transparent solution | 120 | ||||||||
Total | 2592 | Total | 850 |